Selective binding of actinomycin D and distamycin A to DNA
نویسندگان
چکیده
منابع مشابه
Footprinting with MPE.Fe(II). Complementary-strand analyses of distamycin- and actinomycin-binding sites on heterogeneous DNA.
We recently reported a direct technique for determining the binding sites of small molecules on naturally occurring heterogeneous DNA (Van Dyke et al. 1982). Methidiumpropyl-EDTA.Fe(lI) (MPE.Fe[I~) (Hertzberg and Dervan 1982) cleaves double-helical DNA with low sequence-specificity (Van Dyke et al. 1982). Using a combination of MPE-Fe(II) partial cleavage of drugprotected DNA fragments and Maxa...
متن کاملSelective Binding of Distamycin A Derivative to G-Quadruplex Structure [d(TGGGGT)]4
Guanine-rich nucleic acid sequences can adopt G-quadruplex structures stabilized by layers of four Hoogsteen-paired guanine residues. Quadruplex-prone sequences are found in many regions of human genome and in the telomeres of all eukaryotic organisms. Since small molecules that target G-quadruplexes have been found to be effective telomerase inhibitors, the identification of new specific ligan...
متن کاملMap of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA.Fe(II).
We report a direct technique for determining the binding sites of small molecules on naturally occurring heterogeneous DNA. Methidiumpropyl-EDTA.Fe(II) [MPE.Fe(II) cleaves double helical DNA with low sequence specificity. Using a combination of MPE.Fe(II) cleavage of drug-protected DNA fragments and Maxam-Gilbert gel methods of sequence analysis, we have determined the preferred binding sites o...
متن کاملAssociation of actinomycin D and deoxyribodinucleotides as a model for binding of the drug to DNA.
The association of actinomycin D and eleven different deoxyribodinucleotides were studied as model complexes for the interaction of actinomycin D and DNA. All of the deoxyribodinucleotides containing guanine will complex with actinomycin D, and the guanine base has a preferred orientation with respect to the chromophore of actinomycin D. The spectrophotometric binding curve for pdG-dC is sigmoi...
متن کاملSingle-strand DNA binding of actinomycin D with a chromophore 2-amino to 2-hydroxyl substitution.
A modified actinomycin D was prepared with a hydroxyl group that replaced the amino group at the chromophore 2-position, a substitution known to strongly reduce affinity for double-stranded DNA. Interactions of the modified drug on single-stranded DNAs of the defined sequence were investigated. Competition assays showed that 2-hydroxyactinomycin D has low affinity for two oligonucleotides that ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1982
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/10.22.7273